Ref.: MpoBi32-014

Development of a Hybrid Electrode Based on Carbon Paste, Graphene Oxide and Tritrpticin for Biotechnological Applications

Apresentador: Erica Cristina Almeida

Autores (Instituição): Ramos, S.C.(Universidade Estadual do Sudoeste da Bahia); Jesus, R.S.(Universidade Estadual de Santa Cruz); Almeida, E.C.(Universidade Estadual de Santa Cruz); Brasil, M.O.(Universidade Estadual Paulista); Silva, B.L.(Universidade Estadual de Santa Cruz); Cilli, E.M.(Universidade Estadual Paulista); Salay, L.C.(Universidade Estadual de Santa Cruz);

Resumo:
Tritrpticin (Trp3) is a cationic peptide from the cathelicidin family that has large antimicrobial activity and high specificity. Trp3 acts against bacteria and fungi. The aim of this work is the development, characterization, and application of a hybrid electrode (HE) that was formed by a special interaction of Trp3 and graphene oxide (GO). Carbon paste (CP) was used as a supporting material. Electrochemical techniques, mainly cyclic voltage and electrochemical impedance spectroscopy, as well as techniques (spectroscopic fluorescence, UV-visible) were employed to characterize the HETrp3GO. The initial result (FTIR) showed the interaction of Trp3 with GO by amine groups adsorbed on the functionalized surface of GO by oxygen groups. In this sense, HETrp3GO will be used for biological applications such as ascorbic acid (AA) and dopamine (DA) interactions. The first of them acts as an inhibitor of certain cancer types, and the second acts in the central nervous system as a marker for Alzheimer's and Parkinson's diseases. The HETrp3GO can be used as a biosensor for drug (AA, DA) identification, and its quantification could contribute to the biotechnology research area through the possible development of functional polymeric nanocomposites.