Ref.: MCoBi19-003

Optimization of bioinks for 3D bioprinting of smart biomaterials with oxygen delivery property

Apresentador: Wanessa Caroline Alves Santos

Autores (Instituição): Santos, W.C.(Universidade Federal do Piauí); Andrade, H.M.(Universidade Federal do Piauí); Mágulas, M.B.(Universidade Federal do Piauí); Lobo, A.d.(Universidade Federal do Piauí); Marciano, F.R.(Universidade Federal do Piaui); Sousa, G.F.(Universidade Federal do Piauí); Sousa, L.M.(Universidade Federal do Piauí); Lima, G.G.(Universidade Federal do Piauí); Santana, M.d.(Universidade Federal do Piauí); Santos, M.C.(Universidade Federal do Piauí);

Resumo:
Santos, W. C. A.; Mágulas, M. B. S.; Andrade, H. M. A.; Santana, M. V. S.; Lima, G. G.; Santos, M. C.; Sousa, G. F.; Sousa, L. M. R.; Marciano, F. R. Lobo, A. O. According to the Ministry of Health, rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by irreversible joint destruction caused by the proliferation of macrophages and fibroblasts in the synovial membrane after a possibly autoimmune or infectious stimulus [1]. Despite appearing in different age groups, its prevalence is related to the aging process and is considered the most common rheumatic disease among individuals over 65 years of age in Brazil [2]. This disease involves multiple cellular and molecular pathways that converge in the destruction and progressive degradation of cartilage and the cartilaginous matrix, which, in turn, does not have good regenerative capacity as it is a poorly oxygenated tissue due to the lack of vascularization [3]. Aiming to circumvent the degradation process of this tissue, this project focused on improving bioinks for 3D bioprinting using intelligent materials with oxygen delivery properties. Calcium peroxide microparticles (CPO) were obtained by electrospraying technique using polymeric materials such as Poly (butylene adipate-co-terephthalate) (PBAT) and new tests are being carried out with polyvinyl alcohol (PVA). The oxygen release potential of these nanocomposites was measured in an airtight system that mimics human temperature conditions for an average of three days. Next, the microparticles will be incorporated into a hydrogel for 3D bioprinting of structures that will serve as osteochondral scaffolds for damage caused by rheumatoid arthritis. In addition to the analysis of the peroxide produced, the materials will be characterized by: In vitro biological tests; Live/Dead Test; Cell proliferation and differentiation; and real-time PCR.